A multi-component reaction for covalent immobilization of lipases on amine-functionalized magnetic nanoparticles: production of biodiesel from waste cooking oil

Abstract A new approach was used for the immobilization of Thermomyces lanuginosus lipase (TLL), Candida antarctica B (CALB), and Rhizomucor miehei (RML) on amine-functionalized magnetic nanoparticles (Fe 3 O 4 @SiO 2 -NH ) via a multi-component reaction route (using cyclohexyl isocyanide). The method offered single-step very fast process covalent attachment lipases under extremely mild conditions (25 °C, water, pH 7.0). Rapid simple 20 mg RML, TLL, CALB 1 g support produced 100%, 98.5%, 99.2% yields, respectively, after h incubation. immobilized derivatives were then biodiesel production from waste cooking oil. Response surface methodology (RSM) in combination with central composite rotatable design (CCRD) employed to evaluate optimize production. effect some parameters such as catalyst amount, temperature, methanol concentration, water content TLL or water-adsorbent RML CALB, ratio t -butanol (wt%) investigated fatty acid methyl esters (FAME) yield. Graphical